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murine t lymphoblastic cell line bw5147  (ATCC)
94
ATCC murine t lymphoblastic cell line bw5147
Dominant-negative MAML1 peptides specifically suppress growth of human and murine NOTCH1-transformed T-cell lines. (a) Growth suppression of cells expressing the dominant-negative MAML1(13-74)-GFP fusion protein. Each cell line was transduced with MAML1(13-74)-GFP or GFP retrovirus at titers such that only a subpopulation of cells (∼40 to 70%) were transduced. The percentage of GFP+ cells was determined daily by flow cytometry, gating for live cells by forward/side scatter criteria. The percentage of GFP+ cells remaining at each day is expressed as a fraction of the initial (day 3 posttransduction) GFP+ percentage. All cell lines except BJAB were used in three independent experiments; a single representative experiment is shown. (b) Absolute growth rates of SUPT-T1 and <t>BW5147</t> cultures in which >95% of cells had been transduced by the indicated retroviruses. Cell counts were performed daily starting at day 2 postretroviral transduction, and extrapolated cell counts were calculated as described in Materials and Methods. (c) Cell cycle effects of MAML1(13-74)-GFP. DNA content was measured from the SUP-T1 and BW5147 cultures depicted in panel b on day 7 posttransduction (corresponding to day 5 in panel b). Cells were stained with propidium iodide and were analyzed by flow cytometry. MamGFP, MAML1(13-74)-GFP; GFP, GFP only.
Murine T Lymphoblastic Cell Line Bw5147, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hcc 1588  (ATCC)
94
ATCC hcc 1588
Dominant-negative MAML1 peptides specifically suppress growth of human and murine NOTCH1-transformed T-cell lines. (a) Growth suppression of cells expressing the dominant-negative MAML1(13-74)-GFP fusion protein. Each cell line was transduced with MAML1(13-74)-GFP or GFP retrovirus at titers such that only a subpopulation of cells (∼40 to 70%) were transduced. The percentage of GFP+ cells was determined daily by flow cytometry, gating for live cells by forward/side scatter criteria. The percentage of GFP+ cells remaining at each day is expressed as a fraction of the initial (day 3 posttransduction) GFP+ percentage. All cell lines except BJAB were used in three independent experiments; a single representative experiment is shown. (b) Absolute growth rates of SUPT-T1 and <t>BW5147</t> cultures in which >95% of cells had been transduced by the indicated retroviruses. Cell counts were performed daily starting at day 2 postretroviral transduction, and extrapolated cell counts were calculated as described in Materials and Methods. (c) Cell cycle effects of MAML1(13-74)-GFP. DNA content was measured from the SUP-T1 and BW5147 cultures depicted in panel b on day 7 posttransduction (corresponding to day 5 in panel b). Cells were stained with propidium iodide and were analyzed by flow cytometry. MamGFP, MAML1(13-74)-GFP; GFP, GFP only.
Hcc 1588, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hcc 1588/product/ATCC
Average 94 stars, based on 1 article reviews
hcc 1588 - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier

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Dominant-negative MAML1 peptides specifically suppress growth of human and murine NOTCH1-transformed T-cell lines. (a) Growth suppression of cells expressing the dominant-negative MAML1(13-74)-GFP fusion protein. Each cell line was transduced with MAML1(13-74)-GFP or GFP retrovirus at titers such that only a subpopulation of cells (∼40 to 70%) were transduced. The percentage of GFP+ cells was determined daily by flow cytometry, gating for live cells by forward/side scatter criteria. The percentage of GFP+ cells remaining at each day is expressed as a fraction of the initial (day 3 posttransduction) GFP+ percentage. All cell lines except BJAB were used in three independent experiments; a single representative experiment is shown. (b) Absolute growth rates of SUPT-T1 and BW5147 cultures in which >95% of cells had been transduced by the indicated retroviruses. Cell counts were performed daily starting at day 2 postretroviral transduction, and extrapolated cell counts were calculated as described in Materials and Methods. (c) Cell cycle effects of MAML1(13-74)-GFP. DNA content was measured from the SUP-T1 and BW5147 cultures depicted in panel b on day 7 posttransduction (corresponding to day 5 in panel b). Cells were stained with propidium iodide and were analyzed by flow cytometry. MamGFP, MAML1(13-74)-GFP; GFP, GFP only.

Journal:

Article Title: Growth Suppression of Pre-T Acute Lymphoblastic Leukemia Cells by Inhibition of Notch Signaling

doi: 10.1128/MCB.23.2.655-664.2003

Figure Lengend Snippet: Dominant-negative MAML1 peptides specifically suppress growth of human and murine NOTCH1-transformed T-cell lines. (a) Growth suppression of cells expressing the dominant-negative MAML1(13-74)-GFP fusion protein. Each cell line was transduced with MAML1(13-74)-GFP or GFP retrovirus at titers such that only a subpopulation of cells (∼40 to 70%) were transduced. The percentage of GFP+ cells was determined daily by flow cytometry, gating for live cells by forward/side scatter criteria. The percentage of GFP+ cells remaining at each day is expressed as a fraction of the initial (day 3 posttransduction) GFP+ percentage. All cell lines except BJAB were used in three independent experiments; a single representative experiment is shown. (b) Absolute growth rates of SUPT-T1 and BW5147 cultures in which >95% of cells had been transduced by the indicated retroviruses. Cell counts were performed daily starting at day 2 postretroviral transduction, and extrapolated cell counts were calculated as described in Materials and Methods. (c) Cell cycle effects of MAML1(13-74)-GFP. DNA content was measured from the SUP-T1 and BW5147 cultures depicted in panel b on day 7 posttransduction (corresponding to day 5 in panel b). Cells were stained with propidium iodide and were analyzed by flow cytometry. MamGFP, MAML1(13-74)-GFP; GFP, GFP only.

Article Snippet: All other lines were obtained from the American Type Culture Collection (Manassas, Va.), including the murine T-lymphoblastic cell line BW5147 (ATCC designation BW5147.G.1.4).

Techniques: Dominant Negative Mutation, Transformation Assay, Expressing, Transduction, Flow Cytometry, Staining